Invitrogen

CD44 Monoclonal Antibody (IM7.8.1)

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CD44 Antibody (MA5-17875) in Flow

Flow cytometric analysis of Spleen cells stained using anti-CD44/H-CAM Monoclonal Antibody (Product # MA5-17875) at a 0.2 µg/10^6 cells dilution. The Isotype control was a Purified Rat IgG2a

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产品信息

MA5-17875

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

Assay-dependent

流式细胞分析 (Flow)

0.2 µg/1x10^6 cells

查看13篇已发表文章 13篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

其他PubMed (Misc)

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

IM7.8.1

抗原

Mouse CD44

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539259

产品详细信息

MA5-17875 targets CD44/H-CAM in ELISA, FACS, IHC (F), IHC (P), IP, and WB applications and shows reactivity with Mouse samples.

The MA5-17875 immunogen is mouse CD44.

靶标信息

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Chronic Porphyromonas gingivalis lipopolysaccharide induces adverse myocardial infarction wound healing through activation of CD8⁺ T cells.

American journal of physiology. Heart and circulatory physiology

Zaidi Y,Corker A,Vasileva VY,Oviedo K,Graham C,Wilson K,Martino J,Troncoso M,Broughton P,Ilatovskaya DV,Lindsey ML,DeLeon-Pennell KY

MA5-17875 was used in Immunohistochemistry-immunofluorescence to indicate that in response to circulating periodontal endotoxins, memory CD8+ T cells are activated, resulting in an acceleration of macrophage-mediated inflammation after MI.

Mon Nov 01 00:00:00 EDT 2021

Safety of Intraperitoneal Injection of Adipose Tissue-Derived Autologous Mesenchymal Stem Cells in Cats.

Journal of veterinary internal medicine

Parys M,Nelson N,Koehl K,Miller R,Kaneene JB,Kruger JM,Yuzbasiyan-Gurkan V

MA5-17875 was used in flow cytometry to determine the safety of intraperitoneal injection in cats in adipose tissue-derived autologous mesenchymal stem cells

Fri Oct 28 00:00:00 EDT 2016

Developmental dynamics of post-selection thymic DN iNKT.

PloS one

Yassai M,Cooley B,Gorski J

MA5-17875 was used in flow cytometry to study the kinetics of iNKT cell precursors

Tue Jan 29 00:00:00 EST 2013

Cell-to-cell interactions and signals involved in the reconstitution of peripheral CD8 T(CM) and T(EM) cell pools.

PloS one

Zaragoza B,Evaristo C,Kissenpfennig A,Libri V,Malissen B,Rocha B,Freitas AA,Almeida AR

MA5-17875 was used in flow cytometry to determine the reconstitution capacity of transferred isolated naive CD8+ T cells

Mon Mar 14 00:00:00 EDT 2011

Yersinia enterocolitica promotes robust mucosal inflammatory T-cell immunity in murine neonates.

Infection and immunity

Echeverry A,Saijo S,Schesser K,Adkins B

MA5-17875 was used in flow cytometry to investigate the mucosal inflammatory T-cell immunity induced by Yersinia enterocolitica infection

Sun Aug 01 00:00:00 EDT 2010

Studies on the mechanism by which antigen-specific IgG suppresses primary antibody responses: evidence for epitope masking and decreased localization of antigen in the spleen.

Scandinavian journal of immunology

Getahun A,Heyman B

MA5-17875 was used in flow cytometry to study mechanisms of IgG-mediated suppression

Tue Sep 01 00:00:00 EDT 2009

Self-organized criticality theory of autoimmunity.

PloS one

Tsumiyama K,Miyazaki Y,Shiozawa S

MA5-17875 was used in flow cytometry to discuss causes of autoimmunity

Thu Dec 31 00:00:00 EST 2009

Ultraviolet B suppresses immunity by inhibiting effector and memory T cells.

The American journal of pathology

Rana S,Byrne SN,MacDonald LJ,Chan CY,Halliday GM

MA5-17875 was used in flow cytometry to elucidate whether UVB exposure inhibits the expansion of effector CD4+ and CD8+ T cells in skin-draining lymph nodes and reduces the number of CD4+ and IFN-gamma+ CD8+ T cells at sites of challenge

Tue Apr 01 00:00:00 EDT 2008

Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.

Journal of immunology (Baltimore, Md. : 1950)

Wells JW,Cowled CJ,Farzaneh F,Noble A

MA5-17875 was used in flow cytometry to identify key signals required for the exponential expansion of specific CD8 positive mouse T cells in vivo

Mon Sep 01 00:00:00 EDT 2008

Accumulation of effector CD4 T cells during type 2 immune responses is negatively regulated by Stat6.

Journal of immunology (Baltimore, Md. : 1950)

King SB,Knorn AM,Ohnmacht C,Voehringer D

MA5-17875 was used in flow cytometry to investigate the effect of Stat6 in T cell expansion, survival, and recruitment to the lung

Tue Jan 15 00:00:00 EST 2008

Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin.

Cell and tissue research

Kanwar N,Fayyazi A,Backofen B,Nitsche M,Dressel R,von Mollard GF

MA5-17875 was used in flow cytometry to elucidate the role of VAMP8 in the thymus

Sat Nov 01 00:00:00 EDT 2008

Induction of tolerance to innocuous inhaled antigen relies on a CCR7-dependent dendritic cell-mediated antigen transport to the bronchial lymph node.

Journal of immunology (Baltimore, Md. : 1950)

Hintzen G,Ohl L,del Rio ML,Rodriguez-Barbosa JI,Pabst O,Kocks JR,Krege J,Hardtke S,Förster R

MA5-17875 was used in flow cytometry to show that a CCR7-dependent migration of lung dendritic cells to the bronchial lymph node is indispensable for tolerance

Wed Nov 15 00:00:00 EST 2006

Defective CD4+CD25+ regulatory T cell functioning in collagen-induced arthritis: an important factor in pathogenesis, counter-regulated by endogenous IFN-gamma.

Arthritis research & therapy

Kelchtermans H,De Klerck B,Mitera T,Van Balen M,Bullens D,Billiau A,Leclercq G,Matthys P

MA5-17875 was used in flow cytometry to show that the decrease in T regulatory cell activity in collagen-induced arthritis is counter-regulated by endogenous IFN-gamma

Thu Dec 15 00:00:00 EST 2005

IgE enhances antibody and T cell responses in vivo via CD23+ B cells.

Journal of immunology (Baltimore, Md. : 1950)

Getahun A,Hjelm F,Heyman B

MA5-17875 was used in flow cytometry to test if IgE antibodies modulate T helper cells in vivo

Mon Aug 01 00:00:00 EDT 2005

CD8+ immunoregulatory cells in the graft-versus-host reaction: CD8 T cells activate dendritic cells to secrete interleukin-12/interleukin-18 and induce T helper 1 autoantibody.

Immunology

Noble A,Leggat JA,Inderberg EM

MA5-17875 was used in flow cytometry to examine interactions between alloreactive CD4 and CD8 T cells in mixed lymphocyte reactions and in the graft-versus-host reaction.

Fri Aug 01 00:00:00 EDT 2003

A transgenic mouse model genetically tags all activated CD8 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Maris CH,Miller JD,Altman JD,Jacob J

MA5-17875 was used in flow cytometry to track the dynamics of the antiviral CD8+ T cell responses using a transgenic mouse that marks activated T cells.

Mon Sep 01 00:00:00 EDT 2003

Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.

International immunology

Liu L,Rich BE,Inobe J,Chen W,Weiner HL

MA5-17875 was used in flow cytometry to identify the initial source of IL-4 in early immune responses.

Sat Aug 01 00:00:00 EDT 1998

CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19.

Journal of immunology (Baltimore, Md. : 1950)

Sato S,Steeber DA,Jansen PJ,Tedder TF

MA5-17875 was used in flow cytometry to examine the effects of altered signaling thresholds in B lymphocytes using CD19-deficient mice and transgenic mice that expressed human CD19 at varying densities.

Thu May 15 00:00:00 EDT 1997

Targets of p56(lck) activity in immature thymoblasts: stimulation of the Ras/Raf/MAPK pathway.

International immunology

Lin K,Abraham KM

MA5-17875 was used in flow cytometry to demonstrate that the Ras/Raf/MAPK pathway is constitutively activated in lck-transformed immature thymoblasts.

Sat Feb 01 00:00:00 EST 1997

生物信息学

蛋白别名: CD44; CD44 antigen; CD44 molecule; ECMR-III; ECMRIII; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44

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基因别名: AU023126; AW121933; AW146109; Cd44; HERMES; Ly-24; Pgp-1

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UniProt ID: (Mouse) P15379

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Entrez Gene ID: (Mouse) 12505

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功能

hyalurononglucosaminidase activity cytokine receptor activity epidermal growth factor receptor binding protein binding hyaluronic acid binding protein kinase binding cytokine binding phosphoprotein binding transmembrane signal receptor

参与通路

CD44 Monoclonal Antibody (IM7.8.1)

CD44 Antibody (MA5-17875) in Flow

产品信息

MA5-17875

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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