Invitrogen

CD45 Monoclonal Antibody (30-F11), eBioscience™

Name: CD45 Monoclonal Antibody (30-F11), eBioscience\u2122
Brand: Invitrogen
SKU: 14-0451-82

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482 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

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CD45 Antibody (14-0451-82) in Flow

Staining of C57Bl/6 splenocytes with 0.25 µg of Rat IgG2b Isotype Control Purified (Product # 14-4031-82) (open histogram) or 0.25 µg of Anti-Mouse CD45 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Cells in the lymphocyte gate were used for analysis.

CD45 Antibody in Immunohistochemistry (IHC)

CD45 Antibody in Immunocytochemistry (ICC/IF)

CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD45 Antibody in Immunohistochemistry (Paraffin), Immunocytochemistry (IHC (P), ICC/IF)

CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD45 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD45 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD45 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

CD45 Antibody — Figure 4 Regulation of CD45 + cell number by both MEK and Hh signaling pathways. ( A ) Representative immunofluorescent staining images of CD45 + cells in liver tissues after drug treatments. ( B ) Summary of A from 10 images in each group (from at least 3 mice) with the number of CD45 + cells per field under microscope (200x). ( C ) Summary of flow cytometry analysis of CD45+ cells in different treatment groups (from three mice/group). * indicates p < 0.05 and ** indicates a more than additive effect via BLISS independence analysis. {TM}

产品信息

14-0451-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

Assay-Dependent

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免疫组化（石蜡） (IHC (P))

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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酶联免疫吸附实验 (ELISA)

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免疫沉淀 (IP)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

30-F11

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_467251

产品详细信息

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays).

Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

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基因别名: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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功能