Invitrogen

CD45R (B220) Monoclonal Antibody (RA3-6B2), APC, eBioscience™

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225 篇参考文献
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产品信息

17-0452-82

应用
建议稀释比
已发表文章

免疫组化 (IHC)

-
查看5篇已发表文章 5篇已发表文章

免疫组化（冰冻） (IHC (F))

-
查看6篇已发表文章 6篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test
查看213篇已发表文章 213篇已发表文章

酶联免疫吸附实验 (ELISA)

-
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产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Fish, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

推荐的同型对照

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

RA3-6B2

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱 spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_469395

产品详细信息

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; T200

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UniProt ID: (Human) P08575, (Mouse) P06800

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Entrez Gene ID: (Human) 5788, (Mouse) 19264

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功能
protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding phosphoprotein phosphatase activity heparin binding hydrolase activity phosphatase activity heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme
参与通路
negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation activation of MAPK activity positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin leukocyte cell-cell adhesion response to gamma radiation B cell differentiation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion T cell proliferation positive regulation of MAPK cascade positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes negative regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
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