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Invitrogen
CD47 Monoclonal Antibody (miap301), Functional Grade, eBioscience™
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CD47 Antibody (16-0471-81) in Flow
Mouse splenocytes were stained with 0.5 µg of Rat IgG2a kappa Isotype Control (Product # 14-4321-85) (blue histogram) or 0.5 µg of CD47 Monoclonal Antibody, Functional Grade (purple histogram) followed by IgG2a Monoclonal Antibody, PE (Product # 12-4817-82). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
产品信息
16-0471-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
miap301
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta 3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.
No cross-reactivity is seen against human CD47.
Applications Reported: This miap301 antibody has been reported for use in flow cytometric analysis and functional assays (blocking cell aggregation in vitro).
Applications Tested: This miap301 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per million cells in a 100 µL total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
CD47, known as integrin-associated protein (IAP), is a glycosylated transmembrane glycoprotein with five domains, expressed widely across hematopoietic cells like T and B cells, monocytes, platelets, and erythrocytes, as well as non-hematopoietic cells. It interacts with integrins such as CD51/CD61 and CD41/CD61, and serves as a receptor for thrombospondin, mediating bi-directional signaling that affects neural synaptic activity and macrophage phagocytosis. CD47 acts as a ligand for CD172a (SIRP alpha), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. This interaction influences cell migration, B cell adhesion, T cell activation, and neuronal development, particularly in synapse-rich brain and retina regions. It also modulates chondrocyte responses to mechanical signals. T cell expression of CD47 can lead to activation or apoptosis in the presence of thrombospondin. Monoclonal antibody stimulation of CD47 has been shown to induce CD4+CD25- suppressive activity and increase Foxp3 expression. CD47's role in membrane transport, signal transduction, and its broad tissue distribution highlight its significance in various physiological processes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD47; CD47 antigen; IAP; Integrin-associated protein; Leukocyte surface antigen CD47; Rh-related antigen; sCD47; soluble CD 47; soluble CD47
基因别名: 9130415E20Rik; AA407862; AI848868; AW108519; B430305P08Rik; Cd47; IAP; Itgp
UniProt ID: (Mouse) Q61735
Entrez Gene ID: (Mouse) 16423
