Invitrogen

CD6 Recombinant Rabbit Monoclonal Antibody (4H14L18)

Name: CD6 Recombinant Rabbit Monoclonal Antibody (4H14L18)
Brand: Invitrogen
SKU: 702907

高级验证

查看其他44个CD6抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

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CD6 Antibody (702907) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of K-562 (Lane 1), Raji (Lane 2), HT-29 (Lane 3) and MCF-7 (Lane 4). The blot was probed with Anti-CD6 Recombinant Rabbit Monoclonal Antibody (Product # 702907, 1:5,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 72 Da band corresponding to CD6 was observed across the cell lines tested.

Product Image Gallery

CD6 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Anti-CD6 Recombinant Rabbit Monoclonal Antibody (Product # 702907, 1:5000 dilution) shows increased expression of CD6 in Jurkat upon hIL-2, PHA and PMA treatment. {TM}

Immunogen image

Immunogen Image CD6 Recombinant Rabbit Monoclonal Antibody (4H14L18)

产品信息

702907

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4H14L18

抗原

Peptides corresponding to human CD6 [1)aa220-aa237 2)aa641-aa658]

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2762403

产品详细信息

This antibody is predicted to react with Monkey, Dog, Rabbit.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。