Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), Functional Grade, eBioscience™

Name: CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), Functional Grade, eBioscience\u2122
Brand: Invitrogen
SKU: 16-0611-82

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19 篇参考文献

查看其他79个CD61 (Integrin beta 3)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (21)

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow)

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CD61 (Integrin beta 3) Antibody (16-0611-82) in Flow

Staining of mouse bone marrow with Anti-Mouse/Rat CD61 (Integrin beta 3) FITC (left) or PE (right). Appropriate isotype controls were used (open histogram). Cells in the myeloid population were used for analysis.

CD61 (Integrin beta 3) Antibody in Immunocytochemistry (ICC/IF)

CD61 (Integrin beta 3) Antibody in Immunohistochemistry (IHC)

CD61 (Integrin beta 3) Antibody in Western Blot (WB)

CD61 (Integrin beta 3) Antibody in ELISA, Neutralization (ELISA, Neu)

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产品信息

16-0611-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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酶联免疫吸附实验 (ELISA)

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中和实验 (Neu)

Assay-Dependent

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功能检测 (Functional)

Assay-Dependent

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产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2C9.G3

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_468984

产品详细信息

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes.

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.

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