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Invitrogen
CD63 Monoclonal Antibody (H5C6), eFluor™ 660, eBioscience™
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CD63 Antibody (50-0639-42) in Flow
Staining of CD3-IgE+CCR3+ cells from normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (blue) or Anti-Human CD63 eFluor® 660 (purple) (right). Cells in the CD3-IgE+CCR3+ gate were used for analysis (left).
产品信息
50-0639-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
H5C6
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins. CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils. However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes. This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells. Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response.
Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This H5C6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD63, also known as LAMP-3, is a glycoprotein belonging to the tetraspanin family, primarily located in late endosomes, lysosomes, and secretory vesicles across various cell types. It is expressed intracellularly in resting platelets and basophils, but upon activation, CD63 translocates to the plasma membrane, making it a widely used marker for basophil activation. Unlike basophils, mast cells can expose CD63 without activation. CD63 is expressed on activated platelets, monocytes, macrophages, and granulocytes, as well as endothelial cells, fibroblasts, and smooth muscle cells. It interacts with integrins (such as VLA-3 and VLA-6) and TIMP-1, playing roles in mediating allergic responses, phagocytosis, and cell migration. Additionally, CD63 is involved in the regulation of H/K-ATPase trafficking and ROMK1 channels, and serves as a T-cell costimulation molecule. CD63 expression can be used to predict prognosis in early-stage carcinomas and is identical to the melanoma-associated antigen ME491 and the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome, highlighting its significance in both immune responses and disease processes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30
基因别名: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30
UniProt ID: (Human) P08962
Entrez Gene ID: (Human) 967
positive regulation of receptor internalization
platelet degranulation
cell-matrix adhesion
negative regulation of epithelial cell migration
protein transport
cell migration
epithelial cell differentiation
cellular protein localization
endosome to melanosome transport
positive regulation of cell adhesion
pigment granule maturation
regulation of vascular endothelial growth factor signaling pathway
regulation of rubidium ion transport
positive regulation of integrin-mediated signaling pathway
