Invitrogen

CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), Alexa Fluor™ 647, eBioscience™

Name: CD68 Monoclonal Antibody (eBioY1\/82A (Y1\/82A)), Alexa Fluor\u2122 647, eBioscience\u2122
Brand: Invitrogen
SKU: 51-0689-42

高级验证

25个已发表图片

21 篇参考文献

查看其他91个CD68抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

图: 1 / 27

CD68 Antibody (51-0689-42) in Flow

Normal human peripheral blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with Mouse IgG2b kappa Isotype Control, Alexa Fluor 647 (Product # 51-4732-81) (blue histogram) or CD68 Monoclonal Antibody, Alexa Fluor 647 (purple histogram). Cells in the monocyte gate were used for analysis.

CD68 Antibody in Immunocytochemistry (ICC/IF)

CD68 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD68 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD68 Antibody in Immunohistochemistry (IHC)

CD68 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

Product Image Gallery

CD68 Antibody — Antibody clone (eBioY1/82A (Y1/82A)) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in eBioY1/82A (Y1/82A) KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD68 antibody (eBioY1/82A (Y1/82A)). Yellow histogram represents staining with the isotype control. {KO}

产品信息

51-0689-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看12篇已发表文章 12篇已发表文章

免疫电镜技术 (IM)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioY1/82A (Y1/82A)

偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2848399

产品详细信息

Description: The eBioY1/82A antibody reacts with CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (LAMPs) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of cells of mainly myeloid derived cells; monocytes and macrophages, dendritic cells (and in some Langerhans cells), neutrophils, basophils mast cells, myeloid progenitor cells, and a subset of CD34+ hemopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on its homology and structure suggests a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also seen expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL.

Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes while intracellular staining results in about 50% of B cells and most monocytes.

Applications Reported: This eBioY1/82A (Y1/82A) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBioY1/82A (Y1/82A) antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

靶标信息

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。