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CD69 Monoclonal Antibody (FN50), Alexa Fluor™ 561, eBioscience™
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图: 1 / 1
CD69 Antibody (505-0699-42) in Flow
Normal human peripheral blood cells were stimulated overnight with PHA-L (Product # 00-4977-03). Cells were then stained with CD3 Monoclonal Antibody, eFluor 450 (Product # 48-0038-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 561 (Product # 505-4714-81) (left) or CD69 Monoclonal Antibody, Alexa Fluor 561 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50). This data was collected on a 5-laser Cytek Aurora full spectral cytometer.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
505-0699-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
FN50
偶联物
Alexa Fluor™ 561
Alexa Fluor™ 561
Alexa Fluor™ 561
激发/发射光谱
556/569 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.
Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This FN50 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Alexa Fluor 561 emits at 575 nm and is intended for use on spectral cytometers where it may be multiplexed with both PE and PE-eFluor 610.
Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green Laser
靶标信息
CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activation inducer molecule; AIM; BL-AC/P26; C-type lectin domain family 2 member C; CD69; EA1; Early activation antigen CD69; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3
基因别名: CD69; CLEC2C
Entrez Gene ID: (Human) 969
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