Invitrogen

CD7 Monoclonal Antibody (4H9), PerCP-eFluor™ 710, eBioscience™

Name: CD7 Monoclonal Antibody (4H9), PerCP-eFluor\u2122 710, eBioscience\u2122
Brand: Invitrogen
SKU: 46-0078-42

1 篇参考文献

查看其他89个CD7抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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CD7 Antibody (46-0078-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0048-42) and Mouse IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (left) or Anti-Human CD7 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

46-0078-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

4H9

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2016683

产品详细信息

Description: This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation.

CD7 has also been detected on numerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin's lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin's lymphoma.

Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1.

Applications Reported: This 4H9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD7, also known as gp40 or Leu9, is a 40 kDa receptor and member of the immunoglobulin gene superfamily. It features an N-terminal region (amino acids 1-107) that is highly homologous to Ig kappa light chains, while its carboxyl-terminal region is proline-rich, forming a stalk from which the Ig domain projects. CD7 is prominently expressed on the majority of immature and mature T lymphocytes, as well as T cell leukemias. It is also found on natural killer cells, a small subpopulation of normal B cells, and malignant B cells. CD7 plays a crucial role in modulating immune cell activity. Cross-linking of surface CD7 enhances T cell and NK cell functions, as evidenced by increased calcium flux, expression of adhesion molecules, cytokine secretion, and proliferation. CD7 directly associates with phosphoinositol 3-kinase, and its ligation induces the production of D-3 phosphoinositides and tyrosine phosphorylation. The expression of CD7 is an important marker in leukemia diagnostics, highlighting its significance in both normal immune function and disease states.

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