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Invitrogen
CD84 Monoclonal Antibody (2G7), Brilliant Ultra Violet™ 737, eBioscience™
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CD84 Antibody (367-0849-42) in Flow
Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet 737 (Product # 367-4714-81) (blue histogram) or CD84 Monoclonal Antibody, Brilliant Ultra Violet 737 (purple histogram). Viable cells in the monocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
产品信息
367-0849-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
2G7
偶联物
激发/发射光谱
350/740 nm
查看光谱
形式
liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description
The monoclonal antibody 2G7 reacts to CD84, a transmembrane protein of the Ig SLAM superfamily. Expression of CD84 is found on mature B cells, a subset of T cells (mainly CD4+CD45RA+), monocytes and platelets. CD84 is found on a percentage of human hematopoietic progenitor (CD34+) cells. Signaling through CD84 via homotypic interactions involves phosphorylation and recruitment of SAP and EAT-2 leading to production of IFN gamma and T cell activation.
Applications Tested
This 2G7 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity
This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Our internal testing suggests that Brilliant Ultra Violet™ 737 (BUV737) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour.
Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
靶标信息
CD84 is expressed on mature B cells and on B cell lines, including pre B cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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生物信息学
蛋白别名: CD84; CD84 antigen (leukocyte antigen); Cell surface antigen MAX.3; DKFZp781E2378; Hly9-beta; leucocyte differentiation antigen CD84; leukocyte antigen CD84; Leukocyte differentiation antigen CD84; Signaling lymphocytic activation molecule 5; SLAM family member 5
基因别名: CD84; hCD84; LY9B; mCD84; SLAMF5
UniProt ID: (Human) Q9UIB8
Entrez Gene ID: (Human) 8832
adaptive immune response
defense response
homophilic cell adhesion via plasma membrane adhesion molecules
negative regulation of granulocyte macrophage colony-stimulating factor production
negative regulation of interleukin-18 production
negative regulation of mast cell activation
negative regulation of mast cell degranulation
innate immune response
leukocyte migration
regulation of store-operated calcium entry
