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Invitrogen
CD85d (ILT4) Monoclonal Antibody (42D1), Functional Grade, eBioscience™
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CD85d (ILT4) Antibody (16-5149-85) in Flow
Staining of normal human peripheral blood cells with 0.5 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Human CD85d (ILT4) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG PE (Product # 12-4822).Cells in the monocyte gate were used for analysis.
产品信息
16-5149-85
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse,
Non-human primate
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
42D1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody 42D1 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leukocyte Ig-like receptor), MIR (monocytes Ig-like receptor) family. CD85d is a 110 kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally, it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection.
Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux as well as enhancing HLA-G tetramer staining and restoring the stimulating activity of tolerogenic DC.
Applications Reported: This 42D1 antibody has been reported for use in flow cytometric analysis, and funational activity.
Applications Tested: This 42D1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
CD85d, also known as LILRB2 or ILT4, is a member of the immunoglobulin-like transcript (ILT), leukocyte Ig-like receptor (LIR), and monocyte Ig-like receptor (MIR) family. It is a 110 kDa single transmembrane receptor with three Ig-like domains and a long cytoplasmic domain containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs). CD85d is expressed on monocytes, macrophages, and dendritic cells. The receptor binds to non-classical class I molecules such as HLA-G, -B, and G1. Upon ligand binding, the tyrosine phosphatase SHP-1 interacts with the ITIMs, transducing a negative signal that inhibits immune response stimulation. This interaction is thought to contribute to tolerance during fetal implantation and plays a role in controlling inflammatory responses and cytotoxicity, helping to focus the immune response and limit autoreactivity. CD85d is also involved in organ allograft rejection, with the levels of ILT3 and ILT4 on dendritic cells suggesting its role in this process. The LILRB2 gene, located in a gene cluster at chromosomal region 19q13.4, encodes multiple transcript variants with different isoforms, highlighting its complexity and importance in immune regulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD85 antigen-like family member D; CD85d; CD85d antigen; Ig-like transcript 4; ILT-4; Immunoglobulin-like transcript 4; leucocyte Ig-like receptor B2; Leukocyte immunoglobulin-like receptor subfamily B member 2; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2; LIR-2; LIR2CD85D; MIR-10; MIR10LILRA6; Monocyte/macrophage immunoglobulin-like receptor 10; myeloid inhibitory receptor 10
基因别名: CD85D; ILT-4; ILT4; LILRB2; LIR-2; LIR2; MIR-10; MIR10
UniProt ID: (Human) Q8N423
Entrez Gene ID: (Human) 10288
adaptive immune response
negative regulation of antigen processing and presentation
positive regulation of tolerance induction
positive regulation of T cell tolerance induction
immune response-inhibiting cell surface receptor signaling pathway
Fc receptor mediated inhibitory signaling pathway
immune response
cellular defense response
signal transduction
cell surface receptor signaling pathway
cell-cell signaling
positive regulation of interleukin-6 production
heterotypic cell-cell adhesion
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of regulatory T cell differentiation
regulation of immune response
negative regulation of immune response
negative regulation of calcium ion transport
cellular response to lipopolysaccharide
regulation of dendritic cell differentiation
