Invitrogen

CD85j (ILT2) Monoclonal Antibody (HP-F1), Functional Grade, eBioscience™

Name: CD85j (ILT2) Monoclonal Antibody (HP-F1), Functional Grade, eBioscience\u2122
Brand: Invitrogen
SKU: 16-5129-82

3 篇参考文献

查看其他39个CD85j (ILT2)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

CD85j (ILT2) Antibody in Flow Cytometry (Flow)

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CD85j (ILT2) Antibody (16-5129-82) in Flow

Normal human peripheral blood cells were stained with 0.06 µg of Mouse IgG1 kappa Isotype Control (Product # 14-4714-85) (blue histogram) or 0.06 µg of CD85j Monoclonal Antibody, Functional Grade (purple histogram) followed by Anti-IgG1 Monoclonal Antibody, Biotin (Product # 13-4015-82) and Streptavidin, PE (Product # 12-4317-87). Cells in the monocyte gate were used for analysis.

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产品信息

16-5129-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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功能检测 (Functional)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

C. elegans, Human, Non-human primate

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HP-F1

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10669632

产品详细信息

Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F.

The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.

Applications Reported: This HP-F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HP-F1 antibody has been tested by flow cytometric analysis of normal human peripheral cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

CD85j, also known as a member of the leukocyte immunoglobulin-like receptor (LIR) family, is a single transmembrane glycoprotein with a long cytoplasmic domain containing four immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These motifs signal through interactions with SHP-1, transducing negative signals that inhibit immune response stimulation. CD85j is expressed on myeloid cells such as monocytes and dendritic cells, as well as on subsets of lymphoid cells including NK, T, and B cells. The expression of CD85j is correlated with leukemias such as acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL). On CD8+ cells, CD85j expression is associated with effector cell function and plays a significant role in viral infections, including HIV, Epstein-Barr virus, and cytomegalovirus (CMV). CD85j binds to MHC class I molecules, including HLA-G, A, F, B27, E, and F, on antigen-presenting cells. The receptor helps control inflammatory responses and cytotoxicity, focusing the immune response and limiting autoreactivity. CD85j belongs to the subfamily B class of LIR receptors, which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and multiple transcript variants encoding different isoforms have been identified for this gene. Its gene is located in a cluster at chromosomal region 19q13.4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CD85 antigen-like family member J; CD85j; Ig-like transcript 2; ILT-2; Immunoglobulin-like transcript 2; leucocyte Ig-like receptor B1; Leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1; LIR-1; MIR-7; Monocyte/macrophage immunoglobulin-like receptor 7; myeloid inhibitory receptor 7

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基因别名: CD85J; ILT-2; ILT2; LILRB1; LIR-1; LIR1; MIR-7; MIR7; PIR-B; PIRB

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UniProt ID: (Human) Q8NHL6

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Entrez Gene ID: (Human) 10859

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功能

protein phosphatase 1 binding HLA-A specific inhibitory MHC class I receptor activity HLA-B specific inhibitory MHC class I receptor activity MHC class I receptor activity SH2 domain binding MHC class I protein binding protein homodimerization activity immunoglobulin receptor superfamily

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