Invitrogen

CD8a Recombinant Rat Monoclonal Antibody (4SM16)

Name: CD8a Recombinant Rat Monoclonal Antibody (4SM16)
Brand: Invitrogen
SKU: 740029T

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查看其他465个CD8抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (1)

CD8a Antibody in Immunocytochemistry (ICC/IF)

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CD8a Antibody (740029T) in ICC/IF

Immunofluorescent analysis of CD8a was performed using CD8a transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD8a Recombinant Rat Monoclonal Antibody (4SM16) (Product # 740029T, 1:100) followed by Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (Product # A11006, 1:500). Panel a) shows representative images of cells that were stained for ... View More

CD8a Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD8a Antibody — Antibody specificity was demonstrated by relative expression. CD8a Recombinant Rat Monoclonal Antibody (4SM16) (Product # 740029T) specifically detects CD8a expression in mouse colon tissue while no CD8a expression is seen in mouse skeletal muscle tissue. {RE}

产品信息

740029T

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4SM16

抗原

Fragment of extracellular domain

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3679132

产品详细信息

Recombinant Rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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基因别名: BB154331; CD8; CD8A; CD8B; CD8B1; LEU2; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; LY3; Lyt-2; Lyt-3; Lyt2; LYT3; MAL; p32; P37