Invitrogen

CD90 Monoclonal Antibody (F15-42-1)

Name: CD90 Monoclonal Antibody (F15-42-1)
Brand: Invitrogen
SKU: MA5-16671

高级验证

4个已发表图片

8 篇参考文献

查看其他68个CD90 (Thy1)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

CD90 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

CD90 Antibody (MA5-16671) in ICC/IF

Immunofluorescence analysis of CD90 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD90 Monoclonal Antibody (F15-42-1) (Product # MA5-16671) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pr... View More

CD90 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD90 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD90 Monoclonal Antibody (F15-42-1) (Product # MA5-16671), shows relatively higher expression in SH-SY5Y cells as compared to MCF7 cells. {RE}

Immunogen image

Immunogen Image CD90 Monoclonal Antibody (F15-42-1)

产品信息

MA5-16671

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

1:10-1:50

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

Assay-dependent

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Cynomolgus monkey, Human

已发表种属

C. elegans, Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

F15-42-1

抗原

Purified human brain Thy-1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538165

产品详细信息

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is human brain or thymus. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a approximately 25 kDa glycoprotein homologous to rat Thy1.

Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a approximately 25 kDa glycoprotein homologous to rat Thy1.

靶标信息

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。