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Invitrogen
CDC45L Polyclonal Antibody
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FIGURE: 1 / 3
CDC45L Antibody (PA5-79021) in WB
Western blot analysis of CDC45L in Lane 1: rat testis tissue lysate, Lane 2: rat thymus tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: rat lung tissue lysate, Lane 5: mouse testis tissue lysate, Lane 6: mouse thymus tissue lysate, Lane 7: mouse brain tissue lysate, Lane 8: mouse lung tissue lysate, Lane 9: mouse HEPA1-6 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocell... View More
Product Details
PA5-79021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human CDC45L recombinant protein (Position: E166-A431).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat testis tissue, rat thymus tissue, rat brain tissue, rat lung tissue, mouse testis tissue, mouse thymus tissue, mouse brain tissue, mouse lung tissue, mouse HEPA1-6 whole cell.
Target Information
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenylation sites of this gene are reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC 45; CDC 45L; CDC45 cell division cycle 45 homolog; CDC45 like; CDC45-related protein; Cell division control protein 45 homolog; cell division cycle 45 homolog; cell division cycle 45-like 2; human CDC45; PORC PI 1; PORC-PI-1
Gene Aliases: CDC45; CDC45L; CDC45L2; PORC-PI-1; UNQ374/PRO710
UniProt ID: (Human) O75419, (Mouse) Q9Z1X9
Entrez Gene ID: (Human) 8318, (Rat) 287961, (Mouse) 12544
DNA replication checkpoint
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
double-strand break repair via break-induced replication
DNA replication
pre-replicative complex assembly involved in nuclear cell cycle DNA replication
DNA replication initiation
regulation of chromatin silencing at telomere
DNA duplex unwinding
positive regulation of G1/S transition of mitotic cell cycle
mitotic DNA replication preinitiation complex assembly
cell division
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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