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Invitrogen
CDH11 Monoclonal Antibody (5B2H5)
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CDH11 Antibody (32-1700) in WB
Western blot was performed using Anti-CDH11 Monoclonal Antibody (5B2H5)(Product # 32-1700) and a 87kDa band corresponding to CDH11 was observed across positive tissues (Mouse brain and rat brain) tested. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2) and Mouse Skeletal Muscle (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The ... View More
产品信息
32-1700
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Bovine,
C. elegans,
Chicken,
Dog,
Human,
Mouse,
Pig,
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
5B2H5
抗原
Recombinant protein derived from an intracellular sequence of the human Cadherin 11 protein.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is specific for the ~110 kDa Cadherin 11 protein. Cross-reactivity with other Cadherin family members has not been observed. This antibody is confirmed reactive with human and mouse Cadherin 11. Reactivity was confirmed on western blots using MDA.MB231 cells (human) and mouse L fibroblast lysates.
For Immunohistochemistry of frozen sections, incubate 30-60 minutes at room temperature or overnight at 4°C.
靶标信息
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. All cadherins are single-pass transmembrane proteins with a variable number of 110 amino acid extracellular cadherin (EC) domains. Classical cadherins contain five large N-terminal EC domains, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and type II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) cadherin differ from type I cadherins in their specific amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Type II cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin-11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme. Strong expression of Cadherin-11 has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. A variant Cadherin-11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin-11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested but not in non-invasive cell lines, suggesting that Cadherin-11 may be a marker for aggressive, invasive tumor subsets.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cadherin; cadherin 11, type 2, OB-cadherin (osteoblast); Cadherin-11; OB Cadherin; OB-cadherin; OSF-4; Osteoblast cadherin; osteoblast-cadherin
基因别名: Cad-11; CAD11; CDH11; CDHOB; OB; OSF-4
UniProt ID: (Human) P55287, (Mouse) P55288
Entrez Gene ID: (Human) 1009, (Mouse) 12552
