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Invitrogen
CDK9 Recombinant Rabbit Monoclonal Antibody (5H36L13)
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图: 1 / 4
CDK9 Antibody (703404) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized using ice cold 100% Acetone for detection of endogenous CDK9 using Anti-CDK9 Recombinant Rabbit Monoclonal Antibody (Product # 703704, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # 703404, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CDK9 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
703404
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5H36L13
抗原
Protein corresponding to human CDK9 [aa200-aa372]
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Pig, Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Cyclin-dependent kinase 9; RP11-228B15.5; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit
基因别名: CDC2L4; CDK9; PITALRE; TAK
UniProt ID: (Mouse) Q99J95
Entrez Gene ID: (Mouse) 107951
nucleotide binding
DNA binding
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
transcription factor binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
snRNA binding
transcription regulatory region DNA binding
non-receptor serine/threonine protein kinase
DNA repair
regulation of DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
positive regulation of cardiac muscle hypertrophy
phosphorylation
regulation of histone modification
positive regulation of histone phosphorylation
replication fork arrest
positive regulation of transcription from RNA polymerase II promoter
negative regulation of cell cycle arrest
cellular response to cytokine stimulus
negative regulation of mRNA polyadenylation
positive regulation of mRNA 3'-UTR binding
positive regulation of histone H2B ubiquitination
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