Invitrogen

CEP55 Polyclonal Antibody

Name: CEP55 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-29454

高级验证

查看其他12个CEP55抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

CEP55 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

CEP55 Antibody (PA5-29454) in ICC/IF

Detection of differential subcellular localization of CEP55 protein during different phases of mitosis demonstrates antibody specificity. Immunofluorescence analysis using Anti-CEP55 Polyclonal Antibody (Product # PA5-29454), shows its expression at different phases of mitosis in NTERA cells where it is associated with the centrosome throughout the mitotic division. It can be seen at high levels in the condensing chromosomes during Prophase; concentrated near the spindle poles during Metaphase, starts separating in the spindle midzone at A... View More

Product Image Gallery

CEP55 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across different stages of mitosis in NTERA cells. Immunofluorescence analysis using Anti-CEP55 Polyclonal Antibody (Product # PA5-29454), shows clear association of CEP55 with centrosomes throughout mitosis. {RE}

产品信息

PA5-29454

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human CEP55. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.85 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2546930

产品详细信息

Recommended positive controls: IMR32, U87-MG, MCF-7, HeLa.

Predicted reactivity: Rat (80%), Bovine (87%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Plays a role in mitotic exit and cytokinesis (PubMed:16198290, PubMed:17853893). Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (PubMed:17853893). Not required for microtubule nucleation (PubMed:16198290). Plays a role in the development of the brain and kidney (PubMed:28264986). [UniProt]

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。