Invitrogen

CFL2 Monoclonal Antibody (8C13)

Name: CFL2 Monoclonal Antibody (8C13)
Brand: Invitrogen
SKU: MA5-36233

查看其他13个Cofilin 2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (9)

CFL2 Antibody in Immunocytochemistry (ICC/IF)

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CFL2 Antibody (MA5-36233) in ICC/IF

Immunocytochemistry analysis of CFL2 in U20S cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in CFL2 monoclonal antibody (Product # MA5-36233) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

CFL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image CFL2 Monoclonal Antibody (8C13)

产品信息

MA5-36233

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

8C13

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin 2/CFL2 (121-153aa KDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKL).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884067

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human U2OS whole cell, human HepG2 whole cell, human T-47D whole cell, human Raji whole cell, human placenta tissue, human A549 whole cell, rat heart tissue, rat liver tissue, rat kidney tissue, rat brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human lung cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue. ICC/IF: U20S cell. Flow: A549 cell, SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。