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Agrisera
CGL160/ Conserved in green lineage 160 Polyclonal Antibody
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CGL160/ Conserved in green lineage 160 Antibody (AS12-1853) in WB
Respective amounts of µg/µL of chlorophyll from Arabidopsis thaliana leaf extracted with sample buffer (2% SDS, 8% sucrose, 0.2 m M EDTA, 10 m M Tris HCl (p H 6.8) 4% beta-mercaptoethanol) were separated on 15% SDS-PAGE and blotted 1 h to PVDF. Blots were blocked with 10% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:4 000 overnight at 4°... View More
产品信息
AS12-1853
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Part of Arabidopsis thaliana recombinant CGL160.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
CGL160 encodes a nucleus-derived chloroplast protein that localizes to the thylakoid membrane, where it functions as an accessory factor required for efficient accumulation of the chloroplast ATP synthase (CF1CF0) complex. The protein is an integral thylakoid membrane component with a membrane-embedded C-terminal region distantly related to bacterial Atp1/UncI-type assembly factors and an N-terminal, stroma-exposed segment that participates in later assembly steps. Genetic and biochemical analyses in Arabidopsis show that loss of CGL160 markedly reduces thylakoid ATP synthase content while leaving other major photosynthetic complexes largely unchanged, indicating a specific role in ATP synthase biogenesis rather than general thylakoid maintenance. Mechanistically, CGL160 supports formation of the membrane sector and promotes recruitment/association of the soluble CF1 subcomplex to complete CF1CF0 assembly, consistent with observed interactions with CF1 subunits and the accumulation of stromal CF1 intermediates when the N-terminal region is compromised.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT2G31040; AtCGL160; CGL160; CGLD22
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