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Invitrogen
CHCHD10 Polyclonal Antibody
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CHCHD10 Antibody (PA5-143955) in ICC/IF
Immunocytochemistry analysis of CHCHD10 in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CHCHD10 Polyclonal antibody (Product # PA5-143955) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropr... View More
产品信息
PA5-143955
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human CHCHD10 recombinant protein (Position: P80-P142).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hek293 whole cell, monkey heart tissue, monkey liver tissue, human HepG2 whole cell, human A549 whole cell, rat heart tissue, rat liver tissue, rat skeletal muscle tissue lytsates, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue. IHC: human colonic adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue, human ovarian serous adenocarcinoma tissue, human gallbladder adenocarcinoma tissue, rat cardiac muscle tissue. ICC/IF: CACO-2 cell. Flow: THP-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
CHCHD10 encodes a mitochondrial protein that is enriched at cristae junctions in the intermembrane space. It may play a role in cristae morphology maintenance or oxidative phosphorylation. Mutations in this gene cause frontotemporal dementia and/or amyotrophic lateral sclerosis-2. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 7 and 19.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial; Nur77 downstream gene 2; Nur77 downstream protein 2; Protein N27C7-4
基因别名: 1620401E04Rik; AI267078; C22orf16; CHCHD10; FTDALS2; IMMD; N27C7-4; Ndg2; SMAJ
UniProt ID: (Human) Q8WYQ3
Entrez Gene ID: (Human) 400916, (Mouse) 103172, (Rat) 361824
