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Invitrogen
CHD8 Polyclonal Antibody
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FIGURE: 1 / 1
CHD8 Antibody (PA5-140184) in WB
Western blot analysis CHD8 in various lysates. Samples were incubated with CHD8 polyclonal antibody (Product # PA5-140184). Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil.
Product Details
PA5-140184
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human CHD8. Recombinant protein control fragment (Product #RP-109536).
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Immunogen sequence: LDSLTDDSFN QVTQDPIEEA LGLPSSLDSL DQMNQDGGGG DVGNSSASEL VPPPEEAAPT ELSKESTAPA PESITLHDYT TQPASQEQP
Highest antigen sequence indentity to the following orthologs: Rat - 92%, Mouse - 93%.
Target Information
DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase CHD8; axis duplication inhibitor; CHD-8; Chromodomain-helicase-DNA-binding protein 8; duplin; Helicase with SNF2 domain 1
Gene Aliases: AUTS18; CHD8; HELSNF1; KIAA1564
UniProt ID: (Human) Q9HCK8
Entrez Gene ID: (Human) 57680
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
brain development
covalent chromatin modification
negative regulation of Wnt signaling pathway
DNA duplex unwinding
ATP-dependent chromatin remodeling
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase III promoter
digestive tract development
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
negative regulation of fibroblast apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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