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Invitrogen
CHIL3 Polyclonal Antibody
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FIGURE: 1 / 3
CHIL3 Antibody (PA5-81356) in ICC/IF
Immunofluorescence staining of Chi3l3 in RAW246.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CHIL3 Polyclonal Antibody (Product # PA5-81356, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.
Product Details
PA5-81356
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Mouse Chitinase-like 3/Chi3l3 Protein (Met1-Tyr398)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Mouse Chitinase-like 3/Chi3l3.
Target Information
The CHIL3 gene encodes the protein chitinase-like 3, which is primarily secreted by macrophages and is involved in immune regulation, tissue remodeling, and inflammatory responses. CHIL3, also known as Ym1, belongs to the chitinase-like protein family but lacks chitinase activity due to mutations in its catalytic domain. It is predominantly expressed in lung tissue, where it plays a vital role in asthmatic responses and allergic inflammation. Furthermore, CHIL3 exerts anti-inflammatory roles during pathogen infection by modulating macrophage pro-inflammatory activity and promoting the Th2 immune response. Beyond immunological involvement, CHIL3 is integral to tissue repair mechanisms and cancer cell proliferation, contributing to extracellular matrix restructuring and angiogenesis. It facilitates macrophage polarization towards an M2 phenotype, which is associated with tumor progression and metastasis. Thus, CHIL3 is considered a potential biomarker for inflammatory diseases and cancer, with ongoing research examining its therapeutic modulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Beta-N-acetylhexosaminidase Ym1; chitinase 3-like 3; Chitinase-3-like protein 3; Chitinase-like protein 3; ECF-L; Eosinophil chemotactic cytokine; eosinophil chemotactic factor-L; Secreted protein Ym1
Gene Aliases: AI505981; Chi3l3; Chil3; ECF-L; Ym1
UniProt ID: (Mouse) O35744
Entrez Gene ID: (Mouse) 12655
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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