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Invitrogen
CHOP Polyclonal Antibody
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图: 1 / 26
CHOP Antibody (PA5-102305) in IHC (P)
Immunohistochemistry analysis of CHOP in human normal tissues adjacent to colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CHOP polyclonal antibody (Product # PA5-102305) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-102305
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DDIT3(Accession P35638), corresponding to amino acid residues L6-D56.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total DDIT3.
靶标信息
GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C/EBP homologous protein; C/EBP homoologous protein 10; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; DNA damage-inducible transcript 3 protein; Growth arrest and DNA damage-inducible protein GADD153; Growth arrest and DNA-damage-inducible protein GADD153; MGC4154
基因别名: CHOP; CHOP-10; CHOP10; DDIT3; GADD153
UniProt ID: (Mouse) P35639
Entrez Gene ID: (Mouse) 13198, (Rat) 29467
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
cAMP response element binding protein binding
leucine zipper domain binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
RNA polymerase II transcription factor binding
protein homodimerization activity
negative regulation of transcription from RNA polymerase II promoter
blood vessel maturation
transcription, DNA-templated
regulation of transcription, DNA-templated
apoptotic process
ER overload response
response to unfolded protein
cell cycle
cell cycle arrest
Wnt signaling pathway
endoplasmic reticulum unfolded protein response
positive regulation of interleukin-8 production
negative regulation of CREB transcription factor activity
response to endoplasmic reticulum stress
response to starvation
mRNA transcription from RNA polymerase II promoter
positive regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of neuron apoptotic process
regulation of transcription involved in anterior/posterior axis specification
cell redox homeostasis
negative regulation of fat cell differentiation
negative regulation of myoblast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
release of sequestered calcium ion into cytosol
negative regulation of protein kinase B signaling
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
negative regulation of canonical Wnt signaling pathway
positive regulation of neuron death
negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
negative regulation of determination of dorsal identity
response to amphetamine
response to oxidative stress
aging
response to nutrient
response to drug
response to hydrogen peroxide
embryonic organ development
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