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Invitrogen
CKAP5 Monoclonal Antibody (3C13)
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CKAP5 Antibody (MA5-49287) in ICC/IF
Immunocytochemistry analysis of CKAP5 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CKAP5 Monoclonal antibody (Product # MA5-49287) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
产品信息
MA5-49287
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
3C13
抗原
E.coli-derived human ch TOG/CKAP5 recombinant protein (Position: M1-L221).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human U-87MG whole cell, human COLO-320 whole cell, human SW620 whole cell, human HEPG2 whole cell, human CACO-2 whole cell, rat brain tissue, mouse brain tissue. IHC: human liver cancer tissue, human rectal cancer tissue, human renal cancer tissue, rat brain tissue. ICC/IF: Hela cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Colonic and hepatic tumor over-expressed protein (ch-TOG) belongs to the TOG/XMAP215/Dis1 evolutionarily ancient family of microtubule-associated proteins with isoforms found in all major kingdoms of eukaryotes. This protein is required for the assembly and function of the meiotic or mitotic spindles during cell division and is localized to microtubule-organizing centers (MTOCs) during interphase or to the spindle poles and centrosomes during mitosis or cytokinesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Ch-TOG; ch-TOGp; ckap5; colonic and hepatic tumor over-expressed gene protein; Colonic and hepatic tumor overexpressed gene protein; Cytoskeleton-associated protein 5
基因别名: 3110043H24Rik; 4930432B04Rik; ch-TOG; CHTOG; CKAP5; D730027C18Rik; KIAA0097; mKIAA0097; MSPS; T25636; TOG; TOGp
UniProt ID: (Human) Q14008, (Mouse) A2AGT5
Entrez Gene ID: (Human) 9793, (Mouse) 75786, (Rat) 311191
