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Invitrogen
CLCNKB Polyclonal Antibody
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FIGURE: 1 / 5
CLCNKB Antibody (PA5-119867) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of CLCNKB in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with CLCNKB Polyclonal Antibody (Product # PA5-119867) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-119867
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within C-terminal human CLCNKB from aa520-aa687.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Mouse testis tissue lysate, rat testis tissue lysate, MCF-7 cell lysate, human kidney tissue, human seminoma tissue, MG-63, A549.
Subcellular Location: Cell membrane.
Target Information
Chloride channel Kb (CLCNKB) is a member of the CLC family of voltage-gated chloride channels, which comprises at least 9 mammalian chloride channels. Each is believed to have 12 transmembrane domains and intracellular N and C termini. Mutations in CLCNKB result in the autosomal recessive Type III Bartter Syndrome. CLCNKA and CLCNKB are closely related (94% sequence identity), tightly linked (separated by 11 kb of genomic sequence) and are both expressed in mammalian kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chloride channel K1-like; putative basolateral cTAL chloride channel ClC-Ka; chloride channel K2; chloride channel Kb; chloride channel, kidney, B; chloride channel, voltage-sensitive Kb; clC-K2; CLCNK; hClC-Kb; MGC24087; putative basolateral mTAL chloride channel ClC-Ka; RP11-5P18.8; truncated ClC-K2; unnamed protein product
Gene Aliases: ClC-K2; ClC-K2L; Clc-Ka; ClC-Kb; Clck2; CLCKB; Clcnk1l; Clcnk2
UniProt ID: (Human) P51801, (Mouse) Q9WUB6, (Rat) P51802
Entrez Gene ID: (Human) 1188, (Mouse) 56365, (Rat) 79430
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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