Invitrogen

CLOCK Polyclonal Antibody

Name: CLOCK Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-520

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9个已发表图片

16 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

CLOCK Antibody in Immunocytochemistry (ICC/IF)

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CLOCK Antibody (PA1-520) in ICC/IF

Immunofluorescent analysis of CLOCK (green) showing staining in the nucleus and cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CLOCK polyclonal antibody (Product # PA1-520) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More

CLOCK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CLOCK Antibody in Immunohistochemistry (IHC)

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CLOCK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. SATB1 was observed in Mouse Skeletal Muscle but not in Mouse Liver using CLOCK Polyclonal Antibody (Product # PA1-520) in western blot. {RE}

Immunogen image

Immunogen Image CLOCK Polyclonal Antibody

产品信息

PA1-520

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

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免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫组织化学（悬浮细胞） (IHC (Free))

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免疫细胞化学 (ICC/IF)

1:10-1:200

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凝胶阻滞分析 (GS)

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产品规格

种属反应

Hamster, Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: S(11) S I V D R D D S S I F D G L V E E D D(30) C

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2082586

产品详细信息

PA1-520 detects CLOCK from human, mouse, rat and hamster tissues.

PA1-520 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from rat brain homogenate. Immunohistochemical staining of CLOCK in hamster brain with PA1-520 results in the staining of the superchiasmatic nucleus.

PA1-520 immunizing peptide corresponds to amino acid residues 11-30 of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-520 immunizing peptide (Cat. # PEP-072) is available for use in neutralization and control experiments.

靶标信息

This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: bHLHe8; circadian locomoter output cycles kaput; circadian locomoter output cycles kaput protein; Circadian locomoter output cycles protein kaput; Class E basic helix-loop-helix protein 8; clock circadian regulator; clock homolog; hCLOCK; mCLOCK; rCLOCK

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基因别名: 5330400M04Rik; BHLHE8; CLOCK; I79_020252; KAT13D; KIAA0334

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UniProt ID: (Human) O15516, (Mouse) O08785, (Rat) Q9WVS9