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Invitrogen
CNOT3 Polyclonal Antibody
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CNOT3 Antibody (PA5-100231) in ICC/IF
Immunofluorescent analysis of CNOT3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CNOT3 polyclonal antibody (Product # PA5-100231) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100231
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human CNOT3(Accession O75175), corresponding to amino acid residues S258-V308.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total CNOT3.
靶标信息
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; CCR4-NOT transcription complex, subunit 3; Leukocyte receptor cluster member 2; NOT3 (negative regulator of transcription 3, yeast) homolog
基因别名: A930039N10Rik; CNOT3; KIAA0691; LENG2; NOT3; NOT3H
UniProt ID: (Human) O75175, (Mouse) Q8K0V4
Entrez Gene ID: (Human) 4849, (Mouse) 232791, (Rat) 308311
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
nuclear-transcribed mRNA poly(A) tail shortening
trophectodermal cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
negative regulation of translation
gene silencing by RNA
regulation of stem cell population maintenance
regulation of translation
multicellular organism development
