Invitrogen

COMT Monoclonal Antibody (15C10)

Name: COMT Monoclonal Antibody (15C10)
Brand: Invitrogen
SKU: MA5-49153

查看其他24个COMT抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (12)

COMT Antibody in Immunocytochemistry (ICC/IF)

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COMT Antibody (MA5-49153) in ICC/IF

Immunocytochemistry analysis of COMT in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in COMT Monoclonal antibody (Product # MA5-49153) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for ... View More

COMT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-49153

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

15C10

抗原

E.coli-derived human COMT recombinant protein (Position: G52-P271).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074811

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively.

Positive Control - WB: human HepG2 whole cell, human K562 whole cell, human THP-1 whole cell, human HEK293 whole cell, human A549 whole cell, human Caco-2 whole cell, rat RH35 whole cell, mouse Neuro-2a whole cell. IHC: human intestinal cancer tissue, human mammary cancer tissue, human mammary cancer tissue, human placenta tissue, human lung cancer tissue, mouse testis tissue, rat testis tissue. ICC/IF: A431 cell, MCF7 cell. Flow: K562 cell, U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Catechol O-methyltransferase; catechol-O-methyltransferase 1; catechol-O-methyltransferase isoform; catecholamine-O-methyltransferase; epididymis secretory sperm binding protein Li 98n; OTTHUMP00000197749; OTTHUMP00000197750; testicular tissue protein Li 42

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基因别名: COMT; Comt1; D16Wsu103e; D330014B15Rik; HEL-S-98n

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UniProt ID: (Human) P21964, (Mouse) O88587, (Rat) P22734

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Entrez Gene ID: (Human) 1312, (Mouse) 12846, (Rat) 24267

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功能

magnesium ion binding protein binding methyltransferase activity O-methyltransferase activity catechol O-methyltransferase activity transferase activity metal ion binding methyltransferase

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