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CPK-EF/Calcium-dependent protein kinase EF domain Polyclonal Antibody
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CPK-EF/Calcium-dependent protein kinase EF domain Antibody (AS11-1807) in WB
20 µg of total protein from Hordeum vulgare extracted with isolation buffer with 6 M urea were separated on 12% SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was ... View More
产品信息
AS11-1807
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Calcium-dependent protein kinase EF domain.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum
靶标信息
CPK-EF refers to the EF-hand-containing regulatory region characteristic of plant calcium-dependent protein kinases (CPKs), which are cytosolic kinases that often associate with cellular membranes (frequently the plasma membrane) through N-terminal lipid modifications that help position the enzyme near upstream calcium signals and downstream targets. Structurally, a typical CPK is a single polypeptide composed of an N-terminal variable segment, a central serine/threonine protein kinase catalytic domain, an adjacent autoinhibitory/pseudosubstrate segment, and a C-terminal calmodulin-like domain that contains EF-hand motifs (the CPK-EF portion) that bind Ca2+. Ca2+ binding to these EF-hands triggers conformational changes that relieve autoinhibition and activate the kinase, enabling phosphorylation of substrate proteins. Functionally, CPKs act as Ca2+ sensors and signal transducers that couple stimulus-evoked Ca2+ transients to phosphorylation-driven changes in cellular activity, contributing broadly to regulation of plant growth and development and to responses to biotic and abiotic stresses by modulating signaling networks and gene expression programs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtCPK34; CPK34; F7K24.110; F7K24_110
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