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CPK1/Calcium-dependent protein kinase 1 Polyclonal Antibody
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CPK1/Calcium-dependent protein kinase 1 Antibody (AS19-4315) in WB
Plant age: 4 weeks under short-day conditions (8 h light). 20 µg of the total protein from Arabidopsis thaliana plants, (genetic background: Col-0) plants, wild-type (1), cpk 1 (SALK_096452) (2), cpk 1 (SALK_080155 c) (3) were extracted with protein extraction buffer (Durian et al., 2019) and denatured in LaemmLi-sample buffer (final concentrations: 5% (v/v) beta-mercaptoethanol, 69 m M Tris-HCl p H 6.8,11.... View More
产品信息
AS19-4315
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana CPK1 protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
CPK1 encodes a calcium-dependent serine/threonine protein kinase that is predominantly associated with endomembranes, including peroxisomes, with subcellular targeting driven by N-terminal lipidation: an N-terminal Gly required for myristoylation and a nearby Cys that can support palmitoylation, where loss of these acylation sites shifts the protein from peroxisome-associated membranes to the cytosol. Structurally, CPK1 exhibits the canonical CDPK/CPK architecture, comprising an N-terminal variable region followed by a conserved kinase catalytic domain, an autoinhibitory/junction region, and a C-terminal calmodulin-like regulatory domain containing four EF-hand Ca2+-binding motifs that enable direct Ca2+ sensing. Functionally, CPK1 acts as a Ca2+-responsive signaling node that couples stimulus-evoked Ca2+ transients to protein phosphorylation, thereby contributing to regulation of diverse cellular processes in plants; consistent with this role, curated functional annotations support kinase activity and involvement in protein phosphorylation, and experimentally supported localization highlights a membrane-associated pool positioned to decode Ca2+ signals at specific subcellular sites.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G04870; ATCPK1; CALCIUM DEPENDENT PROTEIN KINASE
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