Invitrogen

CRLS1 Polyclonal Antibody

查看其他3个CRLS1抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (4)

CRLS1 Antibody in Immunocytochemistry (ICC/IF)

CRLS1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

CRLS1 Antibody (PA5-100137) in ICC/IF

Immunofluorescent analysis of CRLS1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CRLS1 polyclonal antibody (Product # PA5-100137) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

CRLS1 Antibody in Immunocytochemistry (ICC/IF)

CRLS1 Antibody in Immunocytochemistry (ICC/IF)

CRLS1 Antibody in Western Blot (WB)

CRLS1 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

CRLS1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CRLS1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CRLS1 polyclonal antibody (Product # PA5-100137) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

CRLS1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CRLS1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with CRLS1 polyclonal antibody (Product # PA5-100137) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

CRLS1 Antibody in Western Blot (WB) — Western blot analysis of CRLS1 in various samples (Lane 1: Pc12 treated with blocking peptide, Lane 2: Pc12, Lane 3: 3t3). Samples were incubated with CRLS1 polyclonal antibody (Product # PA5-100137).

CRLS1 Antibody in Western Blot (WB) — Western blot analysis of CRLS1 in mouse heart. Samples were incubated with CRLS1 polyclonal antibody (Product # PA5-100137).

Immunogen image

CRLS1 Polyclonal Antibody

Immunogen Image CRLS1 Polyclonal Antibody

产品信息

PA5-100137

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CRLS1(Accession Q9UJA2), corresponding to amino acid residues M197-A247.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total CRLS1.

靶标信息

The function of this protein is still unknown. The gene of human hypothetical protein (LOC54675) is up-regulated in colon cancer metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Cite this product

生物信息学

蛋白别名: cardiolipin synthetase; CDP-alcohol phosphatidyltransferase; CLS; unnamed protein product

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基因别名: 0610009I22Rik; 4930557M15Rik; 5730490M08Rik; C20orf155; CLS; CLS1; COSPD57; dJ967N21.6; GCD10; RGD1311037

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UniProt ID: (Human) Q9UJA2, (Rat) Q5U2V5, (Mouse) Q80ZM8

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Entrez Gene ID: (Human) 54675, (Rat) 366196, (Mouse) 66586

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1-acylglycerol-3-phosphate O-acyltransferase activity phosphotransferase activity, for other substituted phosphate groups CDP-diacylglycerol-phosphatidylglycerol phosphatidyltransferase activity 2-acylglycerol-3-phosphate O-acyltransferase activity

phospholipid biosynthetic process cardiolipin biosynthetic process response to thyroxine response to phosphatidylethanolamine phosphatidylglycerol acyl-chain remodeling glycerophospholipid biosynthetic process