Invitrogen

CRTC3 Monoclonal Antibody (R.185.4)

Name: CRTC3 Monoclonal Antibody (R.185.4)
Brand: Invitrogen
SKU: MA5-14919

高级验证

查看其他16个CRTC3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (2)

CRTC3 Antibody in Immunocytochemistry (ICC/IF)

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CRTC3 Antibody (MA5-14919) in ICC/IF

Immunofluorescence analysis of Torc3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-Torc3 Rabbit Monoclonal Antibody (Product # MA5-14919) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More

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CRTC3 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target in cell lines owing to their inherent genetic constitution. Differential basal expression of Torc3 was observed in HeLa, U2OS and HEK293 when compared to other cell lines which are reported to have low levels of Torc3 expression using product (Product # MA5-14919) in western blot. {RE}

产品信息

MA5-14919

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

R.185.4

抗原

Synthetic peptide corresponding to the sequence of human TORC3 (CRTC3) protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

168 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10979546

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

The protein encoded by this gene has similarity to a yeast protein. It seems to be part of a peripheral membrane protein complex localized on cis/medial Golgi cisternae where it may participate in tethering intra-Golgi transport vesicles.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CREB regulated transcription coactivator 3; CREB-regulated transcription coactivator 3; TORC-3; transducer of CREB protein 3; transducer of regulated cAMP response element-binding protein (CREB) 3; Transducer of regulated cAMP response element-binding protein 3; transducer of regulated CREB protein 3

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基因别名: 2610312F20Rik; 6332415K15Rik; AI429792; BC011210; CRTC3; RGD1309666; TORC-3; TORC3

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UniProt ID: (Human) Q6UUV7, (Mouse) Q91X84

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Entrez Gene ID: (Human) 64784, (Mouse) 70461, (Rat) 365297

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功能

cAMP response element binding protein binding molecular_function transcription cofactor

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