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Agrisera
CRY1/Cryptochrome 1 Polyclonal Antibody
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CRY1/Cryptochrome 1 Antibody (AS16-3933) in WB
15 µg of total protein from Arabidopsis thaliana etiolated/greening seedling extracted with exact buffer (0.125 M Tris, 1% SDS, 10% Glycerol, 0.05 M Sodium metabisulfite) and denatured at 70°C for 5 min. Samples and Prestained Protein were separated on 10% SDS-PAGE(1.5 mm, 10-well) and blotted 50 min to PVDF using semi-dry. Blots were blocked with 5% nonfat milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for overnight at 4°C with agitation in TBS-T. The antibody solu... View More
产品信息
AS16-3933
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CRY1.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Nicotiana tabacum
靶标信息
Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATCRY1; BLU1; BLUE LIGHT PHOTORECEPTOR; BLUE LIGHT UNINHIBITED 1; CRYPTOCHROME 1 APOPROTEIN; ELONGATED HYPOCOTYL 4; HY4; OOP2; OUT OF PHASE 2; T3H13.14; T3H13_14
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