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Invitrogen
CXCL11 Monoclonal Antibody (10C6)
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CXCL11 Antibody (MA1032) in WB
Western blot analysis of human ITAC was performed by loading 0.25 µg of recombinant human ITAC and induced HeLa cell culture supernatant onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with an anti -human ITAC monoclonal antibody (Product # MA1032 clone 10C6, MA1033 clone 22F1) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA1032
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
10C6
抗原
Full length recombinant protein expressed in E-Coli
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.0 mg/mL
规格
200 µg
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1032 has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MA1033B (biotinylated conjugate of clone 22F1).
Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125-0.25 µg/mL for detection.
The Molecular weight is ~8303 Da from positions 22 - 94 as determined by MALDI.
靶标信息
Interferon-inducible T-cell alpha chemoattractant ( I-TAC), belonging to the CXC chemokine family, is expressed in peripheral blood leukocytes, pancreas and liver, thymus, spleen, lung, small intestine, placenta and prostate. Its molecular weight is 8-11 kD. I-TAC is also known as CXCL-11 and IP-9. The cytokine is involved in regulating calcium release and inducing a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. It is upregulated in patients with AIDS, dementia and in patients with multiple sclerosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; MGC102770
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