Invitrogen

CXCR3 Recombinant Rabbit Monoclonal Antibody (6H1L8)

Name: CXCR3 Recombinant Rabbit Monoclonal Antibody (6H1L8)
Brand: Invitrogen
SKU: 702228

1 篇参考文献

查看其他58个CXCR3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

CXCR3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

CXCR3 Antibody (702228) in ICC/IF

For immunofluorescence analysis, K562 cells were fixed and permeabilized for detection of endogenous CXCR3 using Anti- CXCR3 Recombinant Rabbit Monoclonal Antibody (Product # 702228, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CXCR3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3693... View More

Product Image Gallery

产品信息

702228

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

6H1L8

抗原

Peptides corresponding to Human CXCR3 (aa 21-35, 352-368)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2633069

产品详细信息

This antibody is predicted to react with Monkey, Horse, Goat and Cat

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

CD183, also known as CXCR3, is a seven-transmembrane G protein-coupled chemokine receptor that binds CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (I-TAC), which are part of the CXC chemokine subfamily. CD183 plays a crucial role in leukocyte traffic, influencing integrin activation, cytoskeletal changes, and chemotactic migration. It is expressed on NK cells, subsets of T lymphocytes, regulatory T cells (Tregs), and is preferentially found on Th1-polarized cells. CD183 is prominently expressed in effector/memory T cells and T cells in inflamed tissues, contributing to CD4 T cell responses to grafts, as evidenced by compromised allograft rejection in CXCR3 knockout mice. Chemokine binding induces rapid, short-lived cellular responses due to receptor internalization, with responsiveness restored after receptor recycling. Inhibition by Bordetella pertussis toxin suggests coupling with Gi subclass G proteins. The production of IP-10, Mig, and I-TAC in inflammatory lesions indicates CD183 role in recruiting inflammatory cells, making it a target for developing antagonists to treat inflammatory diseases. Multiple transcript variants encoding different isoforms of CD183 have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: an; C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 3; CC motif chemokine; CCmotif chemokine; CD183; chemokine (C-X-C motif) receptor 3; chemokine receptor 3; CKR-L2; CXC; CXC motif chemokine; CXC-R3; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; IP10; IP10 receptor; Mig receptor

查看更多收起

基因别名: CD182; CD183; CKR-L2; CMKAR3; CXCR3; GPR9; IP10-R; Mig-R; MigR

查看更多收起

UniProt ID: (Human) P49682

查看更多收起

Entrez Gene ID: (Human) 2833

查看更多收起

功能

chemokine receptor activity C-X-C chemokine receptor activity chemokine binding

参与通路