Invitrogen

CYLD Polyclonal Antibody

Name: CYLD Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-29795

高级验证

1 篇参考文献

查看其他16个CYLD抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (5)
高级验证 (1)

CYLD Antibody in Immunocytochemistry (ICC/IF)

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CYLD Antibody (PA5-29795) in ICC/IF

Immunofluorescence analysis of CYLD was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 100% Acetone for 5 minutes, at -20 degree Celsius and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CYLD Polyclonal Antibody (Product # PA5-29795) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 ... View More

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CYLD Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-87 MG cells were transfected with CYLD siRNA and decrease in signal intensity was observed in western blot application using Anti-CYLD Polyclonal Antibody (Product # PA5-29795). {KD}

产品信息

PA5-29795

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human

已发表种属

C. elegans

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the N-terminus region of human CYLD. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.89 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2547269

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (95%), Rat (94%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: cylindromatosis (turban tumor syndrome); Deubiquitinating enzyme CYLD; FLJ20180; FLJ31664; FLJ78684; probable ubiquitin carboxyl-terminal hydrolase CYLD; Ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; Ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; Ubiquitin-specific-processing protease CYLD

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基因别名: BRSS; CDMT; CYLD; CYLD1; CYLDI; EAC; HSPC057; KIAA0849; MFT; MFT1; SBS; TEM; USPL2

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UniProt ID: (Human) Q9NQC7

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Entrez Gene ID: (Human) 1540

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功能

thiol-dependent ubiquitin-specific protease activity protein binding zinc ion binding protein kinase binding Lys63-specific deubiquitinase activity proline-rich region binding ribosomal protein

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