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Invitrogen
CYP27B1 Polyclonal Antibody
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FIGURE: 1 / 5
CYP27B1 Antibody (PA5-26065) in IHC (P)
Immunohistochemistry analysis of CYP27B1 in formalin fixed and paraffin embedded human kidney tissue. Samples were incubated with CYP27B1 polyclonal antibody (Product # PA5-26065) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Product Details
PA5-26065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1alpha(OH)ase; 25 hydroxyvitamin D3-1-alpha hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 25-OHD-1 alpha-hydroxylase; Calcidiol 1-monooxygenase; cy; Cytochrome p450 27B1; cytochrome P450 40 (25-hydroxyvitamin D3 1 alpha-hydroxylase); cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1; Cytochrome P450 subfamily XXVIIB polypeptide 1; cytochrome P450, 40 (25-hydroxyvitamin D3 1 alpha-hydroxylase); cytochrome P450, family 27, subfamily B, polypeptide 1; cytochrome P450, subfamily 27b, polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; P450C1 alpha; P450VD1-alpha; VD3 1A hydroxylase
Gene Aliases: CP2B; CYP1; CYP1ALPHA; CYP27B; CYP27B1; Cyp40; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR
UniProt ID: (Human) O15528, (Rat) O35132
Entrez Gene ID: (Human) 1594, (Rat) 114700
vitamin metabolic process
calcium ion transport
negative regulation of cell proliferation
negative regulation of calcidiol 1-monooxygenase activity
positive regulation of vitamin D 24-hydroxylase activity
bone mineralization
negative regulation of cell growth
regulation of bone mineralization
response to lipopolysaccharide
response to vitamin D
response to interferon-gamma
calcitriol biosynthetic process from calciol
vitamin D metabolic process
vitamin D catabolic process
response to estrogen
positive regulation of keratinocyte differentiation
decidualization
calcium ion homeostasis
oxidation-reduction process
G1 to G0 transition
positive regulation of vitamin D receptor signaling pathway
aging
lactation
response to insulin
response to prostaglandin E
response to drug
response to peptide hormone
response to copper ion
response to cAMP
response to calcium ion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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