Invitrogen

CaV1.3 Monoclonal Antibody (S48), APC

Name: CaV1.3 Monoclonal Antibody (S48), APC
Brand: Invitrogen
SKU: MA5-45391

查看其他26个CaV1.3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

CaV1.3 Antibody in Immunocytochemistry (ICC/IF)

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CaV1.3 Antibody (MA5-45391) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Cav1.3 monoclonal antibody (Product # MA5-45391) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3 antibody (D) Composite.

CaV1.3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image CaV1.3 Monoclonal Antibody (S48), APC

产品信息

MA5-45391

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

S48

抗原

Fusion protein amino acids 859-875 of rat Cav1.3

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偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2931845

产品详细信息

1 µg/mL of MA5-45391 was sufficient for detection of Cav1.3 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 250kDa. No cross-reactivity against Cav1.2.

靶标信息

CACNA1D is also known as PASNA, SANDD or Cav1. 3. Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: brain class D; CACNA 1D; calcium channel alpha-1 subunit; Calcium channel, L type, alpha-1 polypeptide isoform 2; Calcium channel, L type, alpha-1 polypeptide, isoform 2; calcium channel, neuroendocrine/brain-type, alpha 1 subunit; calcium channel, voltage-dependent, L type, alpha 1D subunit; CaV1.3alpha1; RBD; voltage-dependent calcium channel subunit alpha1D; Voltage-dependent L-type calcium channel subunit alpha-1D; voltage-gated calcium channel alpha 1 subunit; voltage-gated calcium channel alpha subunit Cav1.3; voltage-gated calcium channel pore forming subunit CaV1.3alpha1 IVS3-IVS4 extracellular linker; Voltage-gated calcium channel subunit alpha Cav1.3

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基因别名: 8430418G19Rik; C79217; CACH3; CACN4; CACNA1D; CACNL1A2; Cav1.3; Cchl1a; CCHL1A2; D-LTCC; PASNA; SANDD

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UniProt ID: (Human) Q01668, (Mouse) Q99246

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Entrez Gene ID: (Human) 776, (Mouse) 12289, (Rat) 29716

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