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Invitrogen
Caldesmon Polyclonal Antibody
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Caldesmon Antibody (PA5-78906) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Caldesmon in SiHa cells using Caldesmon Polyclonal Antibody (Product # PA5-78906). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 di... View More
产品信息
PA5-78906
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human Caldesmon recombinant protein (Position: M1-E120).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.01mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human U20S whole cell, human A549 whole cell, human HepG2 whole cell, human Hela whole cell, human CACO-2 whole cell, mouse NIH/3T3 whole cell. IHC: mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue. ICC/IF: SiHa cell. Flow: U87 cell.
靶标信息
Caldesmon is a developmentally regulated protein involved in smooth muscle and non muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their electrophorectic mobility and cellular distribution. The h caldesmon variant (120 - 150 kDa) is predominantly expressed in smooth muscle whereas l caldesmon (70 - 80 kDa) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Caldesmon; CDM; h-caldesmon; L-caldesmon; MGC21352; Non-muscle caldesmon; OTTHUMP00000207950; OTTHUMP00000207953; OTTHUMP00000207954; OTTHUMP00000207955; OTTHUMP00000214473; testis secretory sperm-binding protein Li 227n
基因别名: 4833423D12Rik; AI195384; AV071549; AW536160; C920027I18Rik; CAD; CALD1; CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22
UniProt ID: (Human) Q05682, (Rat) Q62736
Entrez Gene ID: (Human) 800, (Rat) 25687, (Mouse) 109624
