Invitrogen

Calnexin Monoclonal Antibody (AF18)

Name: Calnexin Monoclonal Antibody (AF18)
Brand: Invitrogen
SKU: MA3-027

24个已发表图片

81 篇参考文献

查看其他36个Calnexin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (10)
已发表图片 (24)

Calnexin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 34

Calnexin Antibody (MA3-027) in ICC/IF

Immunofluorescent analysis of calnexin (red) in U87 cells. Cell fixed with 4% paraformaldehyde were permeabilized with 0.05% Triton X-100 in PBS for 60 seconds at room temperature and blocked with 5% normal donkey serum for 30 minutes at room temperature. Cells were probed with a calnexin monoclonal antibody (Product # MA3-027) at a dilution of 1:100 for 2 hours at room temperature, washed with PBS, and incubated with a fluorescently-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:100 for 45 minutes at room temperature... View More

Calnexin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Calnexin Antibody in Immunoprecipitation (IP)

Calnexin Antibody in RNA Immunoprecipitation (RIP)

Calnexin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Calnexin Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Immunogen image

Immunogen Image Calnexin Monoclonal Antibody (AF18)

产品信息

MA3-027

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

查看32篇已发表文章 32篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:500

查看37篇已发表文章 37篇已发表文章

免疫沉淀 (IP)

5 µg

查看3篇已发表文章 3篇已发表文章

Proximity Ligation Assay (PLA) (PLA)

查看1篇已发表文章 1篇已发表文章

免疫电镜技术 (IM)

查看1篇已发表文章 1篇已发表文章

RNA免疫共沉淀 (RIP)

Assay-dependent

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Dog, Hamster, Human, Mouse, Non-human primate, Rat, Yeast

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

AF18

抗原

Focus human hepatoma cell lysate.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2069043

产品详细信息

MA3-027 detects calnexin from human and mouse tissues.

MA3-027 has been successfully used in Western blot, immunocytochemistry, immunofluorescence and immunoprecipitation protocols. By Western blot, this antibody detects calnexin in human liver, skin, adrenal gland, heart, colon, testes and ovary extracts. This antibody is not recommended for human kidney or mouse liver lysates in Western blot applications. The predicted molecular weight of this antibody is ~67kDa; however, it may run on SDS-PAGE as an ~90kDA protein. This is due mainly to negative charges at the C-terminus of the protein, which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility. Immunocytochemical staining of calnexin in Huh-7 cells with MA3-027 yields a pattern consistent with specific endoplasmic reticulum staining.

The MA3-027 antigen is Focus human hepatoma cell lysate.

靶标信息

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

仅用于科研。不用于诊断过程。未经明确授权不得转售。