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Invitrogen
Calsequestrin Polyclonal Antibody
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FIGURE: 1 / 4
Calsequestrin Antibody (PA5-85406) in WB
Western blot was performed using Anti-Calsequestrin Polyclonal Antibody (Product # PA5-85406) and a 45 kDa band corresponding to Calsequestrin-1 was observed across tissues tested . Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Rat Heart (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Kidney (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® ... View More
Product Details
PA5-85406
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calsequestrin 1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.79 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (96%), Rat (96%), Pig (96%), Rabbit (96%), Bovine (94%).
Positive Control: HepG2, mouse muscle, rat muscle.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Aspartactin; calmitin; Calmitine; calsequestrin 1 (fast-twitch, skeletal muscle); Calsequestrin, skeletal muscle isoform; Calsequestrin-1; Laminin-binding protein
Gene Aliases: CASQ; CASQ1; CSQ; CSQ-1; CSQ1; PDIB1; sCSQ; VMCQA
UniProt ID: (Human) P31415, (Rat) P19633, (Mouse) O09165
Entrez Gene ID: (Human) 844, (Rat) 686019, (Mouse) 12372
endoplasmic reticulum organization
skeletal muscle tissue development
response to heat
response to organic substance
regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion
response to denervation involved in regulation of muscle adaptation
ion transmembrane transport
sarcomere organization
protein polymerization
regulation of cardiac conduction
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
response to muscle inactivity
regulation of sequestering of calcium ion
regulation of muscle contraction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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