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Invitrogen
Carboxypeptidase Y Monoclonal Antibody (10A5B5)
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Carboxypeptidase Y Antibody (A-6428) in WB
Cells 2024 -
Figure 4 VPS27 deletion impairs the CPY pathway in the sit4 Delta mutant. ( A ) Immunoblotting showing complete processing of CPY in both Log and PDS (24 h after Log) phases in all strains (except in pep4 Delta cells, used as control). ( B ) CPY protein levels were quantified using GAPDH as a loading control. Values are the mean +- SD ( n = 4). ( C ) CPY secretion was assessed using a colony immunoblot assa... View More
产品信息
A-6428
应用
建议稀释比
已发表文章
产品规格
种属反应
Yeast
已发表种属
C. elegans,
Yeast
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
10A5B5
抗原
Purified yeast CPY (carboxypeptidase)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Ig fraction
保存液
HEPES buffered saline
内含物
0.02% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using the yeast caboxypeptidase, by Western blot immunoassay using protein extracts from yeast, and by indirect immunofluorescence of fixed yeast cells.The antibody can be used to detect the yeast carboxypeptidase on Western blots or to immunolocalize the carboxypeptidase in fixed yeast cells. The antibody is ideal for screening for caroboxypeptidase secretion (Vps-phenotype) in yeast using the colony immunoblot overlay assay. The antibody has been extensively used in screens for new vps mutations, as well as in complementation analysis with the vps mutant collection.
靶标信息
Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Carboxypeptidase Y; Carboxypeptidase YSCY; CPD-Y; cpy1; pcy1
基因别名: CPY1; LBC1; PRC1; YMR297W
UniProt ID: (Baker's yeast) P00729
Entrez Gene ID: (Baker's yeast) 855343
