Invitrogen

Caspase 1 (cleaved Asp297) Polyclonal Antibody

Name: Caspase 1 (cleaved Asp297) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-77886

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4 篇参考文献

说明书

实验方案

问题与答案

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说明书

实验方案

问题与答案

技术支持

Caspase 1 (cleaved Asp297) Antibody in Western Blot (WB)

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Caspase 1 (cleaved Asp297) Antibody (PA5-77886) in WB

Knockout of Caspase 1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR917229_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Caspase 1 was performed by loading 30 µg of HT29 Cas9 (Lane 1), HT29 Cas9 treated with 0.1 µg/mL of LPS for 4hrs followed by 1mM of ATP for 2hrs (Lane 2), &(8)203;HT29 CASP1 KO (Lane 3) and... View More

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产品信息

PA5-77886

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:3,000

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亲和纯化 (AP)

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产品规格

种属反应

Human

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human Caspase 1 (cleaved Asp297). The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2735573

产品详细信息

Positive Control: conditioned medium of treated THP-1, THP-1 conditioned medium (500 ng/mL LPS treatment for 4 hr in serum free and 1 mM ATP treatment for 1.5 hr)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Caspase 1 (cleaved Asp297) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 1 (cleaved Asp297) was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. Caspase 1 (cleaved Asp297) has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

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基因别名: CASP1; ICE; IL1BC; IL1BCE; P45

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UniProt ID: (Human) P29466

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Entrez Gene ID: (Human) 834

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功能

endopeptidase activity cysteine-type endopeptidase activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process cysteine-type endopeptidase activity involved in apoptotic process protease

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