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Invitrogen

Caspase 8 (Cleaved Asp384) Polyclonal Antibody

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产品信息

PA5-99435

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:2,000
查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200
-

免疫细胞化学 (ICC/IF)

1:100-1:500
-
产品规格

种属反应

Human

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CASP8(Accession Q14790), corresponding to amino acid residues K367-P394.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

规格

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2818368

产品详细信息

Antibody detects endogenous levels of fragment of activated Caspase 8 resulting from cleavage adjacent to Asp384.

靶标信息

Caspase-8 (cleaved Asp384) refers to the activated form of caspase-8 generated following proteolytic cleavage at aspartic acid residue 384, a key step in the activation of this initiator caspase in the extrinsic apoptosis pathway. Caspase-8 is encoded by the CASP8 gene and is synthesized as an inactive zymogen (pro-caspase-8) containing tandem death effector domains (DEDs) that enable recruitment to death-inducing signaling complexes (DISCs) formed downstream of death receptors such as Fas (CD95), TRAIL receptors, and TNF receptor 1. Upon DISC assembly, pro-caspase-8 undergoes dimerization and autocatalytic cleavage at specific aspartic acid sites, including Asp384, producing active subunits that assemble into the functional enzyme. Activated caspase-8 initiates the apoptotic cascade by cleaving and activating downstream effector caspases such as caspase-3 and caspase-7, and can also process BID to link extrinsic and intrinsic apoptosis pathways. Detection of cleaved caspase-8 (Asp384) is widely used as a marker of death receptor-mediated apoptotic signaling in cell death and cancer research.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase precursor; Caspase-8; Caspase-8 (Asp384 cleaved); Cleaved Caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MORT1-associated ced-3 homolog

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基因别名: ALPS2B; CAP4; Casp-8; CASP8; FLICE; MACH; MCH5

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UniProt ID: (Human) Q14790

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Entrez Gene ID: (Human) 841

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功能
cysteine-type endopeptidase activity death receptor binding tumor necrosis factor receptor binding protein binding peptidase activity cysteine-type peptidase activity hydrolase activity ubiquitin protein ligase binding death effector domain binding identical protein binding macromolecular complex binding scaffold protein binding
参与通路
autophagosome assembly angiogenesis regulation of cytokine production regulation of immune system process proteolysis apoptotic process heart development extrinsic apoptotic signaling pathway via death domain receptors positive regulation of signal transduction regulation of tumor necrosis factor-mediated signaling pathway protein processing natural killer cell activation cell differentiation macrophage differentiation positive regulation of cell migration response to cobalt ion response to estradiol response to lipopolysaccharide positive regulation of interleukin-1 beta production response to tumor necrosis factor TRAIL-activated apoptotic signaling pathway T cell activation B cell activation regulation of apoptotic process chordate embryonic development positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process regulation of innate immune response response to ethanol positive regulation of macrophage differentiation positive regulation of proteolysis animal organ development proteolysis involved in cellular protein catabolic process protein maturation negative regulation of necroptotic process syncytiotrophoblast cell differentiation involved in labyrinthine layer development pyroptosis cellular response to mechanical stimulus response to anesthetic apoptotic signaling pathway extrinsic apoptotic signaling pathway execution phase of apoptosis self proteolysis positive regulation of pyroptotic inflammatory response positive regulation of execution phase of apoptosis
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