Invitrogen

Caspase 9 Polyclonal Antibody

Name: Caspase 9 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-19904

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2个已发表图片

2 篇参考文献

查看其他33个Caspase 9抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Caspase 9 Antibody in Immunocytochemistry (ICC/IF)

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Caspase 9 Antibody (PA5-19904) in ICC/IF

Immunocytochemistry of HeLa cells using Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin... View More

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Immunogen Image Caspase 9 Polyclonal Antibody

产品信息

PA5-19904

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1.0 µg/mL

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免疫细胞化学 (ICC/IF)

5 µg/mL

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A peptide corresponding to amino acids 290 to 340 of human caspase-9.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11153263

产品详细信息

In Western blot applications, this antibody detects a band at ~46 kDa. A suggested positive control is Hela cell lysate.

PA5-19904 can be used with blocking peptide PEP-0030.

靶标信息

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer's Disease (AD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; Caspase-9 precursor; CASPASE-9c; Caspase9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; OTTHUMP00000002324; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3

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基因别名: APAF-3; APAF3; CASP9; ICE-LAP6; MCH6; PPP1R56

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UniProt ID: (Human) P55211

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Entrez Gene ID: (Human) 842

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功能

cysteine-type endopeptidase activity protein binding enzyme activator activity peptidase activity SH3 domain binding protein kinase binding cysteine-type endopeptidase activity involved in apoptotic process protease

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