Invitrogen

Catalase Polyclonal Antibody

Name: Catalase Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95349

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (8)

Catalase Antibody in Immunocytochemistry (ICC/IF)

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Catalase Antibody (PA5-95349) in ICC/IF

Immunocytochemistry analysis of Catalase using anti-Catalase antibody (Product # PA5-95349) . Catalase was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Catalase antibody (Product # PA5-95349) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained ... View More

Catalase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Catalase Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Catalase Polyclonal Antibody

产品信息

PA5-95349

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Catalase recombinant protein (Position: E344-L527).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807152

产品详细信息

Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human A549 whole cell, human HL-60 whole cell, human THP-1 whole cell, human U937 whole cell, human HepG2 whole cell, human K562 whole cell, human HCCT tissue, human HCCP tissue, rat liver tissue, rat kidney tissue, rat lung tissue, rat RH35 whole cell, mouse liver tissue, mouse kidney tissue, mouse lung tissue. IHC: mouse liver tissue, rat liver tissue, human liver cancer tissue. ICC/IF: A431 cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last four amino acids (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3 kDa in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。