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Invitrogen
Cathepsin D Polyclonal Antibody
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FIGURE: 1 / 3
Cathepsin D Antibody (PA5-47046) in ICC/IF
Immunocytochemistry analysis of Cathepsin D in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line. Samples were incubated in Cathepsin D polyclonal antibody (Product # PA5-47046) using a dilution of 5 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to lysoso... View More
Product Details
PA5-47046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin D Ile21-Leu410
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Cathepsin D is observed and less than 1% cross-reactivity with rhCathepsin A, B, C, L, X, and recombinant mouse (rm) Cathepsin B, C, H, and X is observed. In Western blots, approximately 100% cross-reactivity with rhCathepsin D is observed and less than 1% cross-reactivity with rmCathepsin E is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Cathepsin D is a gene located on chromosome 11p15.5 that encodes a lysosomal aspartic protease involved in the degradation of proteins. This enzyme is widely expressed in various tissues but is particularly abundant in the liver, kidneys, and brain, where it plays a crucial role in intracellular protein catabolism and turnover. Cathepsin D is synthesized as an inactive precursor, procathepsin D, which is activated in the acidic environment of lysosomes. Beyond its fundamental role in protein degradation, Cathepsin D has been implicated in several physiological and pathological processes, including apoptosis, hormone processing, and antigen presentation. Dysregulation of Cathepsin D expression or activity is associated with various diseases, such as neurodegenerative disorders, cancer, and cardiovascular diseases. In the context of cancer, Cathepsin D overexpression has been linked to tumor progression and metastasis, making it a potential biomarker and therapeutic target. Research continues to explore its diverse biological functions and therapeutic implications in various medical conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cathepsin D; CathepsinD; Ceroid lipofuscinosis neuronal 10; MGC2311; OTTHUMP00000196039; OTTHUMP00000198692
Gene Aliases: CatD; CD; Ctsd
UniProt ID: (Mouse) P18242
Entrez Gene ID: (Mouse) 13033
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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