Invitrogen

Cathepsin L Monoclonal Antibody (33-2), eBioscience™

Name: Cathepsin L Monoclonal Antibody (33-2), eBioscience\u2122
Brand: Invitrogen
SKU: BMS1032

高级验证

3个已发表图片

7 篇参考文献

查看其他21个Cathepsin L抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Cathepsin L Antibody in Western Blot (WB)

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Cathepsin L Antibody (BMS1032) in WB

Knockout of Cathepsin L was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1042354_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Cathepsin L was performed by loading 30 µg of A549 Cas9 (Lane 1), andA549 Cathepsin L KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More

Cathepsin L Antibody in Immunocytochemistry (ICC/IF)

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产品信息

BMS1032

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

5 µg/mL

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human, Non-human primate

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

33-2

抗原

Rec. Human Cathepsin

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2-7.4

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10596643

产品详细信息

Description: Cathepsin L is a lysosomal cysteine proteinase consisting of a heavy chain of about 25 kDa and a light chain of about 5 kDa derived proteolytically from the same precursor. Cathepsin L has been shown to be produced by many different cell types. Since cathepsin L is capable of degrading protein constituents of the extracellular matrix it is assumed to play a crucial role in tumor progression and metastasis and a number of other disorders where the destruction of the extracellular matrix is the major cause of disease (e.g. rheumatoid arthritis, neurodegeneration).

Applications Tested: ELISA, Immunohistochemistry (Frozen), Western Blotting.

靶标信息

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。